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Investigation of the role of MAPK pathway genes in POP surgical complications
Ahmet Akın SİVASLIOĞLU1, Burcu KASAP1, Melike Nur AKIN1, Eren AKBABA1, Ayşegül DEMİRTAŞ BİLGİÇ2, Melis KILIÇ2, Sevim KARAKAŞ ÇELİK3, Tuba GÖKDOĞAN EDGÜNLÜ41Department of Obstetrics and Gynecology, Muğla Sıtkı Koçman University Faculty of Medicine, Muğla, Türkiye, 2Department of Medical Biology, Muğla Sıtkı Koçman University Institute of Health Sciences, Muğla, Türkiye, 3Department of Medical Genetics, Zonguldak Bülent Ecevit University Faculty of Medicine, Zonguldak, Türkiye, 4Department of Medical Biology, Muğla Sıtkı Koçman University Faculty of Medicine, Muğla, Türkiye
Objectives: We hypothesized that the expressions of genes (JUN, FOS, MAPK1, MAPK8, AKT1) involved in the mitogen-activated protein kinase (MAPK) pathway would change in women with pelvic organ prolapse (POP), and we aimed to elucidate the relationship between this gene and the molecular mechanism of POP. Materials and Methods: A total of 67 cases, including 36 patients (11 mesh, 25 native tissue) and 31 controls obtained from hysterectomy operations, were analyzed in our study. The relationship between MAPK-related genes and POP was investigated using the qRT-polymerase chain reaction method. In addition, we analyzed the genes in silico using Gencodis4 and Genemania web-based tools. Results: The POP patients and control groups were analyzed, and the expression levels of MAPK8 (p=0.036), and AKT1 (p=0.010) genes were significantly higher in the POP group. Also, we have shown that the decreased expression level of the MAPK1 gene was essential in complications (p=0.023). In silico analysis, we determine the biological processes, molecular functions, and biological pathways. Conclusion: We have suggested that MAPK1, MAPK8, and AKT1 genes are effective molecules for POP and POP-related complications. So, in further studies, these genes and related genes may be examined for the determination of the pathophysiological structure of POP disease.
Cite This Article
SİVASLIOĞLU A, KASAP B, AKIN M, AKBABA E, DEMİRTAŞ BİLGİÇ A, KILIÇ M, KARAKAŞ ÇELİK S, GÖKDOĞAN EDGÜNLÜ T. Investigation of the role of MAPK pathway genes in POP surgical complications. 2023; 42(3): 92-98
Corresponding Author: GÖKDOĞAN EDGÜNLÜ T.
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