ABSTRACT

Objectives:

We hypothesized that the expressions of genes (JUN, FOS, MAPK1, MAPK8, AKT1) involved in the mitogen-activated protein kinase (MAPK) pathway would change in women with pelvic organ prolapse (POP), and we aimed to elucidate the relationship between this gene and the molecular mechanism of POP.

Materials and Methods:

A total of 67 cases, including 36 patients (11 mesh, 25 native tissue) and 31 controls obtained from hysterectomy operations, were analyzed in our study. The relationship between MAPK-related genes and POP was investigated using the qRT-polymerase chain reaction method. In addition, we analyzed the genes in silico using Gencodis4 and Genemania web-based tools.

Results:

The POP patients and control groups were analyzed, and the expression levels of MAPK8 (p=0.036), and AKT1 (p=0.010) genes were significantly higher in the POP group. Also, we have shown that the decreased expression level of the MAPK1 gene was essential in complications (p=0.023). In silico analysis, we determine the biological processes, molecular functions, and biological pathways.

Conclusion:

We have suggested that MAPK1, MAPK8, and AKT1 genes are effective molecules for POP and POP-related complications. So, in further studies, these genes and related genes may be examined for the determination of the pathophysiological structure of POP disease.