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Volume : 43 Issue : 1 Year : 2024
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pelviperineology. 2024; 43(1): 12-19 | DOI: 10.34057/PPj.2024.43.01.2023-10-11

Restorative effects of myricetin and hyaluronic acid on vaginal epithelial atrophy in ovariectomized rats

Samet Kadir MUTLU1, Burcu KASAP1, Hülya ELBE2, Dilan ÇETİNAVCI3, Ümmühani ÖZEL TÜRKCÜ4, Melike Nur AKIN1, Ahmet Akın SİVASLIOĞLU1
1Department of Obstetrics and Gynecology, Muğla Sıtkı Koçman University Faculty of Medicine, Muğla, Türkiye, 2Department of Histology and Embryology, Muğla Sıtkı Koçman University Faculty of Medicine, Muğla, Türkiye, 3Clinic of Histology and Embryology, Muğla Training and Research Hospital, Muğla, Türkiye, 4Department of Medical Biochemistry, Muğla Sıtkı Koçman University Faculty of Medicine, Muğla, Türkiye

Objectives: Postmenopausal women often suffer from genitourinary syndrome (GUS), which is vulva-vaginal atrophy. The aim of this study is to evaluate the effect of hyaluronic acid and myricetin on GUS caused by ovariectomy. Materials and Methods: Twenty-eight Wistar Albino female adult rats were randomly divided into 4 equal groups. Vaginal smear and pH were collected from all groups before the procedures. To the vaginal walls, for Sham group (Group 1, n=7): The abdomen was opened and closed, then 0.9% saline/0.5 cc/subcutaneously, for ovariectomy group (Group 2, n=7): Two weeks after ovariectomy, 0.9% saline/0.5 cc/subcutaneously, for ovariectomy + myricetin group (Group 3, n=7): Two weeks after ovariectomy myricetin 5 mg/0.5 cc/subcutaneously, for ovariectomy + hyaluronic acid group (Group 4, n=7): 2 weeks after ovariectomy hyaluronic acid 5 mg/0.5 cc/subcutaneously was applied. After 2 weeks of the injection, vaginal smear and pH were checked again in all rats. Vaginal smears were stained with May-Grunwald Giemsa and Pap. Meisel’s vaginal maturation index was calculated. Vaginectomy was performed. hematoxylin eosin and caspase-3 immunostaining was performed and scored. Biochemically tissue SOD, AOPP, and TSH were measured. Results: Vaginal Maturation Index and pH were significantly increased in group 3 and 4 compared to group 2. Vaginal epithelial thickness was increased in Group 3 and 4 compared to group 2. The vaginal epithelial thickness of group 3 increased more than group 4. No significant change in biochemical parameters was observed between groups. Conclusion: We believe that myricetin will be a promising option for non-hormonal treatment methods in women with GUS symptoms.


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MUTLU S, KASAP B, ELBE H, ÇETİNAVCI D, ÖZEL TÜRKCÜ Ü, AKIN M, SİVASLIOĞLU A. Restorative effects of myricetin and hyaluronic acid on vaginal epithelial atrophy in ovariectomized rats. 2024; 43(1): 12-19

Corresponding Author: MUTLU S.

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